Lab on a Chip Feb. 2014, Vol. 14, No. 4 Inside front cover

�@

�@

Tissue Engineering Part C Oct. 2015, Vol. 21, No.10 Cover art

�@

�@

Biomicrofluidics May 2019, Vol. 13, No. 3 Front cover Biomicrofluidics Best Paper Award 2019

1. Original Papers

40) Kiyonori Noda and Hidehiro Oana,
�gToward single-cell epigenome analysis: A microfluidic device for isolating, stretching, and imaging individual chromosomes,�h
Sensors and Actuators B: Chemical, 394, Article number: 134462 (7 pages) (2023).

39) K. O. Okeyo, R. Hiyaji, H. Oana,
�gA single-cell surgery microfluidic device for transplanting tumor cytoplasm into dendritic cells without nuclei mixing,�h
Biotechnology Journal, e2200135 (9 pages) (2023).

38) Y. Ma, Y. Yoshikawa, H. Oana, K. Yoshikawa,
�gMarked Difference in the Conformational Transition of DNA Caused by Propanol Isomer,�h
Polymers, 12, 1607 (12 pages) (2020).

37) M. Okanojo, K. O. Okeyo, H. Hanzawa, O. Kurosawa, H. Oana, S. Takeda, M. Washizu,
�gNuclear transplantation between allogeneic cells through topological reconnection of plasma membrane in a microfluidic system,�h
Biomicrofluidics 13, Article number: 034115 (12 pages) (2019).

36) T. Takahashi, K. O. Okeyo, J. Ueda, K. Yamagata, M. Washizu, H. Oana,
�gA microfluidic device for isolating intact chromosomes from single mammalian cells and probing their folding stability by controlling solution conditions,�h
Scientific Reports, 8, Article number: 13684 (10 pages) (2018).

35) K. Hayakawa, K. Terada, T. Takahashi, H. Oana, M. Washizu, S. Tanaka,
�gNucleosomes of polyploid trophoblast giant cells mostly consist of histone variants and form a loose chromatin structure,�h
Scientific Reports, 8, Article number: 5811 (15 pages) (2018).

34) K. O. Okeyo, M. Tanabe, O. Kurosawa, H. Oana, M. Washizu,
�gSelf�]organization of human iPS cells into trophectoderm mimicking cysts induced by adhesion restriction using microstructured mesh scaffolds,�h
Development Growth & Differentiation, 60, 183-194 (2018).

33) H. Mori, K. O. Okeyo, M. Washizu, H. Oana,
�gNucleosomes Exhibit Non-uniform Unwrapping Along Native Chromatin Fibers with Increasing Salt Concentration as Revealed by Direct Imaging in a Microfluidic Channel,�h
Biotechnology Journal, 13, 1700245 (8 pages) (2018).

32) K. O. Okeyo, O. Kurosawa, H. Oana, H. Kotera, M. Washizu,
�gMinimization of cell-substrate interaction using suspended microstructured meshes initiates cell sheet formation by self-assembly organization,�h
Biomedical Physics & Engineering Express, 2, 065019 (14 pages) (2016).

31) S. Sakamoto, K. O. Okeyo, S. Yamazaki, O. Kurosawa, H. Oana, H. Kotera, M. Washizu,
�gAdhesion patterning by a novel air-lock technique enables localization and in-situ real-time imaging of reprogramming events in one-to-one electrofused hybrids,�h
Biomicrofluidics, 10, 054122 (13 pages) (2016).

30) K. Terao, C. Masuda, R. Inukai, M. Gel, H. Oana, M. Washizu, T. Suzuki, H. Takao, F. Shimokawa, and F. Oohira,
�gCharacterization of Optically Driven Microstructures for Manipulating Single DNA Molecules under a Fluorescence Microscope,�h
IET Nanobiotechnology, 10, 124-128 (2016).

29) K. O. Okeyo, O. Kurosawa, S. Yamazaki, H. Oana, H. Kotera, H. Nakauchi, and M. Washizu,
�gCell Adhesion Minimization by a Novel Mesh Culture Method Mechanically Directs Trophoblast Differentiation and Self-Assembly Organization of Human Pluripotent Stem Cells,�h
Tissue Engineering Part C, 21, 1105-1115 (2015).

28) Hidehiro Oana, Kaori Nishikawa, Hirotada Matsuhara, Ayumu Yamamoto, Takaharu G. Yamamoto, Tokuko Haraguchi, Yasushi Hiraoka, and Masao Washizu,
�gNon-destructive handling of individual chromatin fibers isolated from single cells in a microfluidic device utilizing an optically driven microtool,�h
Lab on a Chip, 14, 696-704 (2014).

27) Hidehiro Oana*, Mutsuki Morinaga, Akihiro Kishimura*, Kazunori Kataoka, Masao Washizu,
�gDirect Formation of Giant Unilamellar Vesicles from Microparticles of Polyion Complexes and Investigation of Their Properties Using a Microfluidic Chamber,�h
Soft Matter, 9, 5448-5458 (2013).

26) Yuji Kimura, Yuya Goto, Hidehiro Oana, Masao Washizu,
�gOptical Sequence Probing with the Homologous Recombination Protein RecA,�h
Journal of Biotechnology, 164, 254-259 (2012).

25) Takuya Saito, Takahiro Sakaue, Daiji Kaneko, Masao Washizu, and Hidehiro Oana,
�gFolding dynamics of tethered giant DNA under strong flow,�h
J. Chem. Phys. 135, 154901 (2011),
selected for the October 31, 2011 issue of Virtual Journal of Nanoscale Science& Technology.

24) Yuji Kimura, Murat Gel, Boonchai Techaumnat, Hidehiro Oana, Hidetoshi Kotera, Masao Washizu,
�gDielectrophoresis-assisted massively parallel cell pairing and fusion based on field constriction created by a micro-orifice array sheet,�h
Electrophoresis, 32, 2496-2501 (2011).

23) Murat Gel, Yuji Kimura, Osamu Kurosawa, Hidehiro Oana, Hidetoshi Kotera, and Masao Washizu,
�gDielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array,�h
Biomicrofluidics 4, 022808 (2010).

22) Murat Gel, Shohei Suzuki, Yuji Kimura, Osamu Kurosawa, Boonchai Techaumnat, Hidehiro Oana, and Masao Washizu,
�gMicroorifice-based high-yield cell fusion on microfluidic chip: electrofusion of selected pairs and fusant viability,�h
IEEE TRANSACTIONS ON NANOBIOSCIENCE, 8, 300-3005 (2009).

21) Hidehiro Oana*, Akihiro Kishimura*, Kei Yonehara, Yuichi Yamasaki, Masao Washizu, and Kazunori Kataoka,,
�gSpontaneous Formation of Giant Unilamellar Vesicles from Microdroplets of Polyion Complex via Thermally Induced Phase Separation,�h
Angewandte Chemie International Edition, 48, 4613-4616 (2009).

20) B. Techaumnat, K. Tsuda, O. Kurosawa, M. Gel, H. Oana, and M. Washizu,
�gHigh-yield electrofusion of biological cells based on field tailoring by microfabricated structures,�h
IET Nanobiotechnology, 2, 93-99 (2008).

19) K. Terao, M. Washizu and H. Oana,
�gOn-site manipulation of single chromosomal DNA molecules by using optically driven microstructures,�h
Lab on a Chip, 8, 1280-1284 (2008).

18) O. Kurosawa, H. Oana, S. Matsuoka, A. Noma, H. Kotera, and M. Washizu,
�gElectroporation through a micro-fabricated orifice and its application to the measurement of cell response to external stimuli,�h
Meas. Sci. Technol., 17, 3127-3133 (2006).

17) H. Oana, K. Kubo, K. Yoshikawa, H. Atomi, and T. Imanaka,
�gOn-site manipulation of single whole-genome DNA molecules using optical tweezers,�h
Appl. Phys. Lett., 85, 5090-5092 (2004).

16) Y. Yoshikawa, M. Suzuki, N. Chen, A. A. Zinchenko, S. Murata, T. Kanbe, T. Nakai, H. Oana, K. Yoshikawa,
�gAscorbic acid induces a marked conformational change in long duplex DNA,�h
Eur. J. Biochem. 270, 3101-3106 (2003).

15) H. Oana, K. Tsumoto, Y. Yoshikawa, K. Yoshikawa,
�gFolding transition of large DNA completely inhibits the action of a restriction endonuclease as revealed by single-chain observation,�h
FEBS Lett., 530, 143-146 (2002).

14) S. S. Abramchuk, A. R. Khokhlov, T. Iwataki, H. Oana and K. Yoshikawa,
�gDirect observation of DNA molecules in a convection flow of a drying droplet,�h
Europhys. Lett., 55, 294-300 (2001).

13) H. Mayama, S. M. Nomura, H. Oana and K. Yoshikawa,
�gSelf-Oscillating Polymer Chain,�h
Chem. Phys. Lett., 330, 361-367 (2000).

12) H. Oana, M. Ueda, and M. Washizu,
�gVisualization of a Specific Sequence on a Single Large DNA Molecule Using Fluorescence Microscopy Based on a New DNA-Stretching Method,�h
Biochem. Biophys. Res. Commun., 265, 140-143 (1999).

11) J. J. Schwinefus, R. W. Hammond, H. Oana, S.-C. Wang, O. de Carmejane, J. Bonadio, and M. D. Morris,
�gComparative Conformational Dynamics of Supercoiled Plasmids and Linear DNA during Capillary Electrophoresis,�h
Macromolecules, 32, 4625-4630 (1999).

10) M. Ueda, H. Oana, Y. Baba, M. Doi, K. Yoshikawa,
�gElectrophoresis of Long DNA Molecules in Linear Polyacrylamide Solutions,�h
Biophysical Chemistry, 71, 113-123 (1998).

9) H. Oana, R. W. Hammond, J. J. Schwinefus, S.-C. Wang, M. Doi, M. D. Morris,
�gHigh Speed Separation of Linear and Supercoiled DNA by Capillary Electrophoresis. Buffer, Entangling Polymer and Electric Field Effects,�h
Analytical Chemistry, 70, 574-579 (1998).

8) H. Oana, M. Doi, M. Ueda, K. Yoshikawa,
�gReorientation of Large DNA Molecules in Concentrated Polyacrylamide Solution during Crossed Field Electrophoresis,�h
Electrophoresis, 18, 1912-1915 (1997).

7) R. W. Hammond, H. Oana, J. J. Schwinefus, J. Bonadio, R. J. Levy, M. D. Morris,
�gCapillary Electrophoresis of Supercoiled and Linear DNA in Dilute Hydroxyethyl Cellulose Solution,�h
Analytical Chemistry, 69, 1192-1196 (1997).

6) Y. Masubuchi, H. Oana, M. Matsumoto, M. Doi,
�gBrownian Dynamics Simulation of Biased Sinusoidal Field Gel Electrophoresis,�h
Macromolecules, 30, 912-918 (1997).

5) H. Oana, Y. Masubuchi, M. Matsumoto, M. Doi, K. Yoshikawa,
�gMotion of Large DNA Molecules Traveling from Solution to Gel Under Steady Field,�h
Journal of Polymer Science: Part B: Polymer Physics, 34, 1105-1111 (1996).

4) Y. Masubuchi, H. Oana, M. Matsumoto, M. Doi, K. Yoshikawa,
�gConformational dynamics of DNA during biased sinusoidal field gel electrophoresis,�h
Electrophoresis, 17, 1065-1074 (1996).

3) Y. Masubuchi, H. Oana, T. Akiyama, M. Matsumoto, M. Doi,
�gDynamics of a DNA Molecule Hanging over an Obstacle in Gel Electrophoresis,�h
Journal of the Physical Society of Japan, 64, 1412-1420, (1995).

2) H. Oana, Y. Masubuchi, M. Matsumoto, M. Doi, Y. Matsuzawa, K. Yoshikawa,
�gPeriodic Motion of Large DNA Molecules during Steady Field Gel Electrophoresis,�h
Macromolecules, 27, 6061-6067 (1994).

1) Y. Masubuchi, H. Oana, K. Ono, M. Matsumoto, M. Doi, Y. Matsuzawa, K. Minagawa, K. Yoshikawa,
�gPeriodic Behavior of DNA Molecules during Steady Field Gel Electrophoresis,�h
Macromolecules, 26, 5269-5270 (1993).

2. Proceedings/Abstracts

52) K. Noda, H. Oana,
�gMOORING BOTH ENDS OF INTACT CHROMATIN FIBERS TO MICROSTRUCTURES IN A MICROFLUIDIC DEVICE FOR ACQUISITION OF EPIGENETIC INFORMATION BASED ON FLUORESCENCE MICROSCOPY,�h
Proc. 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021), 517-518 (2021).

51) K. O. Okeyo, O. Kurosawa, H. Oana, M. Washizu,
�gMODULATING THE CELL ADHESION MICROENVIRONMENT TO MECHANICALLY DRIVE TROPHECTODERM-LIKE ORGANOID FORMATION FROM HUMAN iPS CELLS,�h
Proc. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019), 290-291 (2019).

50) K. O. Okeyo, K. Yamada, O. Kurosawa, H. Oana, M. Washizu,
�gReconstituting a Bilayer Cellular System Using an Artificial Matrigel Basement Membrane Achieved by the Micromesh Technique�h
2018 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2018).

49) M. Okanojo, K. O. Okeyo, H. Hanzawa, O. Kurosawa, H. Oana, S. Takeda, M. Washizu,
�gNUCLEAR TRANSPLANTATION BETWEEN ALLOGENEIC CELLS ACHIEVED BY FUSION-BASED TOPOLOGICAL RECONNECTION OF THE PLASMA MEMBRANE IN A MICROFLUIDIC SYSTEM,�h
Proc. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), 1425-1427 (2018).

48) K. O. Okeyo, K. Yamada, O. Kurosawa, H. Oana, M. Washizu,
�gRECONSTITUTION OF AN EPITHELIAL-ENDOTHELIAL BILAYER BY THE MICROMESH CULTURE TECHNIQUE EMPLOYING AN ARTIFICIAL MATRIGEL BASEMENT MEMBRANE,�h
Proc. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), 1617-1619 (2018).

47) H. Oana, T. Takahashi, K. O. Okeyo, J. Ueda, M. Washizu,
�gDirect Observation-based Analysis of Compaction Stability of Individual Chromosomes Isolated from Single Mammalian Cells,�h
Proc. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017), 116-117 (2017).

46) Kai Yamada, Kennedy O. Okeyo, Osamu Kurosawa, Hidehiro Oana, and Masao Washizu,
�gToward Fabrication of Vascularized Tissues using a Bilayer Co-culture Approach Achived by a Mesh Culture Technique,�h
2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 293-296 (2017).

45) T. Takahashi, K. O. Okeyo, M. Washizu, J. Ueda, H. Oana,
�gDirect Acquisition of Genome-wide Epigenetic Information along Intact Chromatin Fibers of Individual Chromosomes Isolated from Single Mammalian Cells,�h
Proc. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), 182-183 (2016).

44) S. Sakamoto, K. O. Okeyo, O. Kurosawa, H. Oana, and M. Washizu,
�gLive Imaging of Somatic Nuclear Reprogramming using an Electrofusion Microfluidic Device with Air-lock Patterned Adhesion Areas for Fusant Localization,�h
Proc. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), 371-372 (2016).

43) K. O. Okeyo, M. Azeyanagi, O. Kurosawa, H. Oana, and M. Washizu,
�gFabrication of Freely Standing Oriented Myotubes using a Novel Mesh Culture Method and Analysis of Calcium Dynamics for Functionality Assay,�h
Proc. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), 439-440 (2016).

42) T. Takahashi, K. O. Okeyo, M. Washizu, J. Ueda and H. Oana,
�gDirect Observation of Epigenetic Modifications along Intact Chromatin Fibers of Individual Chromosomes Isolated from Single Cells in a Microfluidic Channel,�h
JSAP 2016 International Conference on Solid State Devices and Materials (SSDM 2016), 407-408 (2016).

41) Kennedy O. Okeyo, Osamu Kurosawa, Satoshi Yamazaki, Hidehiro Oana, Hidetoshi Kotera, and Masao Washizu,
�gCell Adhesion Control using Microstructured Meshes Induces Self-Assembly-Mediated Organoid Formation by Human iPS Cells,�h
2015 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2015), 176-178,
selected as Best Paper Award.

40) Kennedy O. Okeyo, Osamu Kurosawa, Satoshi Yamazaki, Hidehiro Oana, Hidetoshi Kotera, and Masao Washizu,
"Cell Adhesion Control by a Novel Mesh Culture Method Directs Differentiation and Self-assembly of Human iPS Cells into Trophoblasts: Towards Organ-on-Chip Embryogenesis,�h
Proc. 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015), 573-575 (2015).

39) Hiroki Mori, Kennedy O. Okeyo, Masao Washizu and Hidehiro Oana,
"Mapping Positional Distribution of Higher-order Structures along Unfragmented Native Chromatin Fibers Isolated from Single Cells in a Microchannel,�h
Proc. 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015), 1259-1261 (2015).

38) S. Sakamoto, K. O. Okeyo, O. Kurosawa, H. Oana, H. Kotera and M. Washizu,
"Narrowing Slit Device for High Yield One-to-One Electrofusion and Culture of Fusants without Gene Mixing,�h
2014 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2014), 291-294 (2014).

37) Y. Itagaki, K. O. Okeyo, O. Kurosawa, H. Oana, M. Narita, H. Kotera, and M. Washizu,
�gCYTOPLASMIC TRANSFER WITHOUT NUCLEI MIXING BETWEEN DENDRITIC CELLS AND TUMOR CELLS ACHIEVED BY ONE-TO-ONE ELECTROFUSION VIA MICRO-ORIFICES IN A MICROFLUIDIC DEVICE,�h
Proc. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), 814-816(2014).

36) K. O. Okeyo, T. Isozaki, O. Kurosawa, H. Oana, H. Kotera, and M. Washizu,
�gSTABLE AND LONG TERM CULTURE OF STEM CELLS UNDER SHEAR FLOW ON A MICROSTRUCTURED MESH SHEET EMBEDDED IN A FLUIDIC CHAMBER,�h
Proc. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), 907-909 (2014).

35) K. O. Okeyo, R. Yanaru, O. Kurosawa, H. Oana, H. Kotera, and M. Washizu,
�gGENERATION OF EPITHELIAL CELL SHEETS WITH DEFINED CELL ORIENTATION USING MICROSTRUCTURED MESH SHEETS AS SUBSTRATES FOR CELL CULTURE,�h
Proc. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), 1128-1130 (2014).

34) D. Sueyoshi, A. Kishimura, H. Oana, Y. Anraku, M. Takai, M. Washizu, and K. Kataoka,
�gMICROCHANNEL-ASSISTED PREPARATION OF POLYION COMPLEX VESICLES AND REAL-TIME OBSERVATION OF THEIR DYNAMIC RESPONSES TO EXTERNAL ELECTRIC FIELDS,�h
Proc. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), 1182-1184 (2014).

33) K. O. Okeyo, Y. Hayashi, O. Kurosawa, H. Oana, H. Kotera, M. Washizu,
�gON-CHIP ELECTROPORATION DEVICE FOR DIRECT INTRODUCTION OF PLASMIDS INTO CELL NUCLEUS AND OBSERVATION OF CELL REPROGRAMMING PROCESS,�h
Proc. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), 113-115 (2013).

32) K. O. Okeyo, N. Omasa, O. Kurosawa, H. Oana, H. Kotera, M. Washizu,
�gCELL ADHESION CONTROL INITIATE CELL SHEET FORMATION IN A MEDIUM SUSPENSION,�h
Proc. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), 1057-1059 (2013).

31) H. Oana, K. Nishikawa, H. Matsuhara, A. Yamamoto, T. G. Yamamoto, T. Haraguchi, Y. Hiraoka, and M. Washizu,
�gNON-INVASIVE HANDLING OF CHROMATIN FIBERS ISOLATED FROM INDIVIDUAL CELLS IN A MICROCHANNEL UTILIZING AN OPTICALLY DRIVEN MICROTOOL �|TOWARD DIRECT EPIGENETIC ANALYSIS BY MICROSCOPY�|,�h
Proc. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), 1995-1997 (2013).

30) H. Oana, T. Shino, K. Nishikawa, and M. Washizu,
"Real-Time FISH using Optically-driven Microspheres Functionalized by the Homologous Recombination Protein, RecA,"
Proc. 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012), 770-772 (2012).

29) H. Oana, M. Morinaga, A. Kishimura, M. Gel, K. Kataoka, and M. Washizu
"FORMATION OF POLYMER VESICLES FROM MICRODROPLETS OF POLYION COMPLEX AND EXAMINATION OF THEIR PHYSICOCHEMICAL PROPERTIES IN MICROFLUIDIC CHAMBER"
Proc. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011), 1588-1590 (2011).

28) K. Nishikawa, M. Kataoka, R. Nagata, A. Kitayama, R. Tero, M. Washizu, and H. Oana
"EXTENSION, IMMOBILIZATION AND CHEMICAL MODIFICATION OF DOUBLE-STRANDED DNA ON A SOLID SURFACE - TOWARD DIRECT SEQUENCING WITH MICROSCOPY -"
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2010), 82-84 (2010).

27) O. Kurosawa, Y. Sumita, M. Gel, H. Oana, H. Kotera, T. Kato�C J. Toguchida, and M. Washizu
"DIRECT INTRODUCTION OF PLASMID INTO NUCLEUS USING ON-CHIP ELECTROPORATION"
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2010), 217-219 (2010).

26) Y. Kimura, Y. Nishigaichi, Y. Nakada, Y. Mori, H. Iwanari, M. Gel, O. Kurosawa, H. Oana, T. Hamakubo, H. Kotera, and M. Washizu
"SIZE-INDEPENDENT ELECTRO CELL FUSION WITH MASSIVE PARALLELISM"
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2010), 693-695 (2010).

25) M. Gel, Y. Kimura, S. Suzuki, O. Kurosawa, H. Oana, H. Kotera, and M. Washizu
"MICRO ORIFICE BASED HIGH YIELD CELL-CELL FUSION: ON-CHIP ANALYSIS OF POST-FUSION PHENOMENA"
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2010), 956-958 (2010).

24) H. Oana, A. Kishimura, Y. Yamasaki, M. Washizu, and K. Kataoka
"Spontaneous Formation of Giant Unilamellar Vesicles from Microdroplets of a Polyion Complex by Focused Infrared Laser Irradiation"
2009 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2009), 155-160 (2009).

23) M. Gel, Y. Mori, Y. Kimura, O. Kurosawa, B. Techaumnat, H. Oana, M. Washizu
"Micro orifice based cell pairing and fusion on microfluidic chip"
2009 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2009), 517-520 (2009).

22) Y. Goto, Y. Kimura, H. Oana, and M. Washizu
�gNovel DNA optical mapping method using homologous recombination protein, RecA�h
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009), 1527-1529 (2009).

21) Y. Sato, M. Gel, K. Terao, T. Kanai, H. Atomi, T. Imanaka, M. Washizu, H. Oana
�gSPACE-RESOLVED DETECTION OF PROTEIN BINDING SITES ON GENOMIC DNA BY DYNAMIC STRETCHING USING OPTICALLY DRIVEN MICROSTRUCTURES�h
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009), 1368-1370 (2009).

20) M. Gel, K. Hirano, S. Suzuki, Y. Kimura, O. Kurosawa, H. Oana, H. Kotera, T. Tada, M. Washizu
�gMICRO-ORIFICE BASED CELL FUSION APPLIED FOR THE CREATION OF CYTOPLASMIC HYBRID CELLS WITHOUT GENE MIXING�h
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009), 654-656 (2009).

19) O. Kurosawa, H. Oana, H. Kotera, T. Kato, J. Toguchida, and M. Washizu
"MASSIVELY PARALLEL ON-CHIP ELECTROPORATION DEVICE DESIGNED FOR LONG TERM POST-CULTURING"
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009), 570-572 (2009).

18) T. Saito, T. Sakaue, D. Kaneko, M. Washizu, and H. Oana
"Dynamics in the Folding of Long DNA Chain under Strong Flow"
2008 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2008), 118-123 (2008).

17) Y. Kimura, M. Gel, B. Techaumnat, O. Kurosawa, K. Tsuda, H. Oana, H. Kotera, T. Tada, and M. Washizu
"A Novel Cell Manipulation Device for Cytoplasmic Transplantation"
2008 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2008), 196-201 (2008).

16) H. Oana, K. Yonehara, A. Kishimura, Y. Yamasaki, K. Kataoka, and M. Washizu
"SPONTANEOUS FORMATION OF POLYMER VESICLES FROM MICRODROPLETS OF POLYION COMPLEX VIA MICROPHASE SEPARATION"
Proc. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2008), 1, 631-633 (2008).

15) Y. Kimura, M. Gel, B. Techaumnut, K. Tsuda, H. Oana, H. Kotera, T. Tada, and M. Washizu
"HIGH-YIELD PARALLEL ELECTRO-FUSION DEVICE BASED ON FIELD CONSTRICTION AT AN ORIFICE ARRAY"
Proc. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2008), 1, 540-542 (2008).

14) Y. Mori, H. Oana, H. Atomi, T. Imanaka, M. Washizu
"Visualization of an oriC region on an isolated single whole-genome DNA with triplex forming PNA probe using fluorescence microscopy"
2007 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2007), 79-84 (2007).

13) Rikiya Watanabe, Hidehiro Oana and Masao Washizu
"Ligation of DNA based on single-molecule manipulation"
2007 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2007), 85-90 (2007).

12) H. Oana, M. Ohuchi and M. Washizu
"Controlling higher-order structures of giant genomic DNA molecules in microfluidic channel"
Proc. 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), 1, 197-199 (2007).

11) Rikiya Watanabe, Hidehiro Oana and Masao Washizu
"Single-molecule ligation of DNA as a unit operation for molecular surgery"
Proc. 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), 1, 412-414 (2007)

10) K. Terao, H. Oana, M. Washizu
"Isolation of single chromosomal DNA molecule under microscope using optically-driven microbobbins"
Proc. 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), 2, 1000-1002 (2007).

9) Kinya Tsuda, Murat Gel, Hidehiro Oana, Boonchai Techaumnat, Hidetoshi Kotera and Masao Washizu
"Very high yield electro cell-fusion based on field constriction at an microorifice"
Proc. 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), p.1375-1377 (2007).

8) Murat Gel, Osamu Kurosawa, Hidehiro Oana and Masao Washizu
"Fabrication and operation of a micro orifice array chip with high electroporation efficiency"
Proc. 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), 512-514 (2007).

7) K. Terao, H. Kabata, H. Oana, M. Washizu
"Complete extension of chromosomal DNA and its manipulation using optically-driven micro-structures"
2006 IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2006), 1: 47-51 (2006).

6) Masafumi Fuke, Osamu Kurosawa, Hidehiro Oana and Masao Washizu
�gDNA NANO-STAMPING FOR MOLECULAR SURGERY�h
Proc. 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2006), 1, 16-18 (2006).

5) Osamu Kurosawa, Hidehiro Oana, Hidetoshi Kotera and Masao Washizu
�gHIGH-YIELD ELECTROPORATION USING RF-MODULATED FIELD CONSTRICTION�h
Proc. 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2006), 1, 458-460 (2006).

4) K. Terao, H. Kabata, H. Oana, M. Washizu
"Manipulation of yeast chromosomal DNA using optically-driven microstructures"
Proceedings of SPIE (Optical Trapping and Optical Micromanipulation III), 6326: 632626 (2006).

3) K. Terao, H. Kabata, H. Oana and M. Washizu
"COMPLETE EXTENSION OF CHROMOSOMAL DNA AND ITS MANIPULATION USING OPTICALLY-DRIVEN MICRO-FABRICATED HOOKS"
Proc. 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2005), 1, 715-717 (2005).

2) M. Washizu, Y. Wake, O. Kurosawa, H. Oana, S. Matsuoka, A. Noma, H. Kotera
"HIGH-YIELD ELECTROPORATION OF CELLS USING FIELD CONSTRICTION AT MICRO ORIFICES"
Proc. 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2005), 2, 1401-1403 (2005).

1) H. Oana, I. Hagiya, M. Washizu, K. Kubo, K. Yoshikawa, H. Atomi, and T. Imanaka
"Isolation of genomic DNA molecule from a single cell and control its higher order structure using optical tweezers"
Proc. SPIE (Optical Trapping and Optical Micromanipulation II), 5930, 543-550 (2005).